Composite

Part:BBa_K1222000

Designed by: Tingling Ke   Group: iGEM13_TzuChiU_Formosa   (2013-09-23)


pCam (T7 promoter+lac operator+CamR antisense"2"+T7 terminator+Lac promoter+LacI)

This part is IPTG inducible for regulating the production of CamR antisense.We have designed four sets of CamR antisense to inhibit CamR.(BBa_K1222984, BBa_K1222983, BBa_K1222982, BBa_K1222981)but we have only shown one(CamR antisense 2) of the four antisense as the other three are relatively similar just the CamR antisense is different.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/apCam (T7 promoter+lac operator+CamR antisense"2"+T7 terminator+Lac promoter+LacI)